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Get PriceIn addition to grinding, a combination of other lysis treatments was tested, including sonication (with the Cup Horn device; protocol 4b) and thermal shocks (see Materials and Methods). After extraction, aliquots that theoretically should contain 25 to 150 ng of lambda DNA were analyzed by gel electrophoresis (data not shown).
Get Price2021-5-1 · When one or more high-energy laser pulses of femtosecond (10 −15 s) to microsecond (10 −6 s) duration impact the surface of a solid target sample, a breakdown occurs leading to removal, i.e. ablation, if the pulse irradiance exceeds a threshold value that depends on the aggregation state of the material (Radziemski and Cremers, 1989). If the kinetic energy imparted …
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Get Price2020-5-19 · Throughout the four months (56 sampling days), only S. aemula was visited, and on only five days at three sites. It should be noted that S. aemula was the only plant that flowered throughout the survey season; the other three were restricted to the first month (only D. revoluta had some flowers still present in December).
Get PricePrincipal component analysis showed that this elevation might be related to the debromination of BDE-209 during the thermal treatment process of products when …
Get Price2014-3-14 · air-to-alr thermal resistance : r~sIstance (f) a la transmission de chaleur, r4slstance (f) ~ la transmission thermlque air velocity : vltesse (f) de l'alr
Get Price2015-7-1 · Averaged population responses measured as impulses/s (imp/s) of 14 and 31 monkey dopamine neurons, respectively. [From Fiorillo et al. ( 160 ).] B: detection response, and possibly also second response component, enhanced by stimulus …
Get PriceTo prepare one cup of green tea, usually between 1.8 and 3 g of tea is used per cup (Kaegi, 1998; Blumenthal, 2003; Henning et al., 2003; Gruenwald et al., 2004). In Japan and China, 2–3 g loose tea leaves or tea bags are typically used for tea brewing in 100–150 mL water, sometimes repeatedly, while American tea drinkers typically use 2.25 ...
Get PriceDimethyl sulfoxide is a 2-carbon sulfoxide in which the sulfur atom has two methyl substituents.It has a role as a polar aprotic solvent, a radical scavenger, a non-narcotic analgesic, an antidote, a MRI contrast agent, an Escherichia coli metabolite and an alkylating agent.
Get Price2013-4-15 · Regression analysis using sampling interval and timing as explanatory variables explained 26.6% of the variation in TE D /TE C. If, however, the sampling interval is restricted to ≤7 d and the multiple linear regression is performed using the same explanatory variables, the R 2 improves to 0.427 (data not shown). A large fraction of the ...
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Get PriceDimethyl sulfoxide is a 2-carbon sulfoxide in which the sulfur atom has two methyl substituents.It has a role as a polar aprotic solvent, a radical scavenger, a non-narcotic analgesic, an antidote, a MRI contrast agent, an Escherichia coli metabolite and an alkylating agent.
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Get Price2021-4-13 · As pain perception is a highly complex sensory and subjective emotional experience generated in the brain ( IASP, 2020 ), quantifying noxious-evoked brain activity may represent a better proxy pain measure, and a more sensitive marker of analgesic efficacy, compared with reflex signals generated by the spinal cord.
Get Price2021-5-7 · Seven samples from the eDNA sampling of the historical locations, out of 161 samples, and 1 from the eDNA sampling of high climatic suitability, out of 80 samples, were inhibited prior to cleanup. The r 2 for the Sierra Nevada yellow-legged frog assay standard curve ranged from 0.989–1.000 with efficiency from 94.2–115.8%.
Get PriceChoice of extraction buffer. A TENP buffer (50 mM Tris, 20 mM EDTA, 100 mM NaCl, 1% [wt/vol] polyvinylpolypyrrolidone) developed by Picard et al. was used.Similar buffers have later been used by others (3, 18, 60) for extraction of soil DNA.Tris and EDTA protect the DNA from nuclease activity, NaCl provides a dispersing effect, and polyvinylpolypyrrolidone absorbs humic acids and …
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Get Price2011-5-5 · Sampling locations and geochemical analysis Vents and vent fields were located from global information system coordinates established from past USGS surveys (for example, Morgan et al., 1977 , 2007 ).
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Get Price2014-3-14 · analysis and have led to further IEA sponsored research. Annex V Air Infiltration Centre ... Norway, Sweden, Switzerland, United Kingdom and United States of America. (iv) INTRODUCTION This document is intended for use as a supplement to AIRGLOSS ... (S)/thermlque (S)) air-to-alr thermal resistance : r~sIstance (f) a la transmission de chaleur ...
Get PriceEdmonds TE, Hepher MJ, West TS. Thermal cycling for piezoelectric gas detector systems Analytica Chimica Acta. 187: 293-299. DOI: 10.1016/S0003-2670(00)82921-9 : 1: 1986: Bain DC, Berrow ML, McHardy WJ, Paterson E, Russell JD, Sharp BL, Ure AM, West TS. Optical, electron and x-ray spectrometry in soil analysis Analytica Chimica Acta. 180: 163
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Get PriceIn recent years, several protocols based on the extraction of nucleic acids directly from the soil matrix after lysis treatment have been developed for the detection of microorganisms in soil. Extraction efficiency has often been evaluated based on the recovery of a specific gene sequence from an organism inoculated into the soil. The aim of the present investigation was to improve …
Get Price2021-3-12 · Flavo-diiron proteins (FDPs) function as anaerobic nitric oxide scavengers in some microorganisms, catalyzing reduction of nitric to nitrous oxide. The FDP from Thermotoga maritima can be prepared in a deflavinated form with an intact diferric site (deflavo-FDP). Hayashi et al. [(2010) Biochemistry 49, 7040–7049] reported that reaction of NO with reduced deflavo-FDP …
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Get Price2019-4-10 · 48 Department of Astronomy, School of Physics, Peking University, Beijing 100871, People's Republic of China 49 Kavli Institute for Astronomy and Astrophysics, Peking University, Beijing 100871, People's Republic of China 50 Instituto Nacional de Astrofísica, Óptica y Electrónica. Apartado Postal 51 y 216, 72000. Puebla Pue., México
Get PriceEdmonds TE, Hepher MJ, West TS. Thermal cycling for piezoelectric gas detector systems Analytica Chimica Acta. 187: 293-299. DOI: 10.1016/S0003-2670(00)82921-9 : 1: 1986: Bain DC, Berrow ML, McHardy WJ, Paterson E, Russell JD, Sharp BL, Ure AM, West TS. Optical, electron and x-ray spectrometry in soil analysis Analytica Chimica Acta. 180: 163
Get PriceAbstract. Colour morphs sometimes have different behavioural strategies which may be maintained by frequency or density dependence mechanisms. We investigated temporal changes in behavioural reaction to a novel environment among colour morphs (yellow, orange, white) of the European wall lizard (Podarcis muralis).Adult males were given two 15 min experimental trials, and their …
Get Price2 天前 · SW-846 is organized into chapters providing guidance on how to use the methods and groups of methods, called “series”, which are organized by topic. The methods and chapters change over time as updates are published to keep up with evolving analytical and measurement needs. In addition to the methods that are in the official SW-846 ...
Get Price2016-7-12 · Our data suggest that the sudden rise in atmospheric temperature during the Paleocene–Eocene transition was not accompanied by highly elevated carbon dioxide concentrations >∼2,500 ppm. Instead, the low 13C/12C isotope ratios during the Paleocene–Eocene Thermal Maximum were most likely caused by a significant contribution of methane to the …
Get Price&EFA United States Environmental Protection Agency Industrial Environmental Research Laboratory Cincinnati OH 45268 EPA-600/9-8C June 1980 Research and Development Oil Shale Symposium Sampling, Analysis and Quality Assurance March 1979 ----- RESEARCH REPORTING SERIES Research reports of the Office of Research and Development, U.S. Environmental ...
Get Price2019-4-10 · 48 Department of Astronomy, School of Physics, Peking University, Beijing 100871, People's Republic of China 49 Kavli Institute for Astronomy and Astrophysics, Peking University, Beijing 100871, People's Republic of China 50 Instituto Nacional de Astrofísica, Óptica y Electrónica. Apartado Postal 51 y 216, 72000. Puebla Pue., México
Get Price2019-8-14 · Caretta caretta is the most common sea turtle species in the Mediterranean Sea. The species is threatened by anthropomorphic activity that causes thousands of deaths and hundreds of strandings along the Mediterranean coast. Stranded turtles are often cared for in rehabilitation centres until they recover or die. The objective of this study was to characterize the gut …
Get PriceNot mentioned (‘Medium-flow sampling systems’) 15 l min −1 1 week Bello et al. (2018) • Aerosol dust: IOM inhalable sampler (25-mm quartz filter) • CIP 10-M rotating cup bioaerosol sampler with solution of butyl benzoate containing 5-mM 1-(9-anthracenylmethyl) piperazine • …
Get Price2016-12-13 · Cells can use a process known as macropinocytosis to take up fluid from their surroundings. This process plays an important role in many situations. For example, it allows human immune cells to sample their environment to search for harmful microbes and viruses and helps cancer cells to collect more nutrients so that they can grow more rapidly.
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Get PricePerforming challenge tests on RTE products should be highly recommended, also in order to generate data for the production and validation of predictive models, as valuable tool to be used in risk analysis activities. 5 CULTURE MEDIA. Aloa (Agar Listeria according to Ottaviani and Agosti) (BD Difco Oxford, United Kingdom).
Get PriceIn order to assess sand transport modelling impacts a saltation threshold shear stress (τ_ crit) of 0.050 N m-2 is estimated using Bagnold's equation; τ _crit = A[gD(ρ s − ρ)], where A is a constant (0.1 for fluid threshold), ρ s is the mineral grain density (2660 kg m-3), g is the gravitational acceleration (9.81 m s-2), and D is the ...
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Get Price2020-4-3 · The target population contains members of a group that a researcher is interested in studying. The results of the study are generalized to this population, because they all have significant traits in common. The 'sample' is different. This is the actual group from which data is collected. It is a subset of the population and should be a typical ...
Get Price2002-1-2 · used in water analysis. This chapter is the second of the series in water analysis. Each chapter is published individually and is for sale by the Superintendent of Documents, U.S. Government Printing Office, Washington, D.C. 20402. III
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Get Price2015-6-1 · About 20 g sub samples of muscles tissues were taken and digested in a Teflon cup with concentrated HNO 3 at 105 ± 20°C for 8 hours and frozen before analysis. The powdered samples were digested, and the digests kept in sterile plastic bottles prior to analysis. 18 The concentration of Pb and Hg were determined using ICP-AES and a mercury ...
Get Price2011-5-5 · Microbial community analysis: pyrosequencing. Diversity analysis in this study focused on only the >0.1–0.8<μm size class. For a single 1/2 plate 454-FLX pyrosequencing run, a …
Get Price2020-7-1 · (b) Theorized antecedents of tightness. Past research has shown that ecological threat—broadly defined as inclusive of social and environmental threats [2–5]—is a major antecedent of cultural tightness in contemporary countries and states [3,4].Two of the tightest countries are India—which spends a large share of its budget fighting natural disasters []—and …
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Get Price2021-5-5 · This Company Admits That A Preloaded App Went Rogue. Even as Chinese owned TV brands are steadily gaining traction in many markets around the works, including India and North America, there is a ...
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Get Price2015-7-1 · Averaged population responses measured as impulses/s (imp/s) of 14 and 31 monkey dopamine neurons, respectively. [From Fiorillo et al. ( 160 ).] B: detection response, and possibly also second response component, enhanced by stimulus …
Get Price2014-7-21 · The program for the thermal cycling targeting the Lactobacillus spp. group was run as described by Karlsson et al. (Reference Karlsson, Molin, Cilio and Ahrne 2011), and was as follows: 95°C for 5 min, followed by 40 cycles with denaturation at 95°C for 5 s, annealing and elongation at 60°C for 20 s. The fluorescent products were detected at ...
Get Price2021-7-26 · Selected publications: Masuku B, Mkhwanazi N, Young E, Koch A, Warner DF. (2018) Beyond the lab: Eh!woza and knowing tuberculosis. Med Humanit. 44:285-292.. Moosa A, Lamprecht DA, Arora K, Barry CE 3rd, Boshoff HIM, Ioerger TR, Steyn AJC, Mizrahi V, Warner DF. (2017) Susceptibility of Mycobacterium tuberculosis cytochrome bd oxidase mutants to …
Get Price2018-3-28 · Abstract. Polycotylidae is a clade of plesiosaurians that appeared during the Early Cretaceous and became speciose and abundant early in the Late Cretaceous. However, this radiation is poorly understood. Thililua longicollis from the Middle Turonian of Morocco is an enigmatic taxon possessing an atypically long neck and, as originally reported ...
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Get Price2018-9-19 · Within the scope of the 3Rs of Russel and Burch, the number of laboratory animals can be reduced by repeated use of an animal. This strategy only becomes relevant, if the total amount of pain, distress or harm the individual animal experiences does not exceed the severity of a single manipulation. For example, when using imaging techniques, an animal can be examined several …
Get Price2017-3-17 · O14 Thermal dose effects by MR-guided focused ultrasound on the pig brain tissue - preliminary results Dong-Guk Paeng 1,3, Zhiyuan Xu 2, John Snell 1, Anders H. Quigg 1, Matthew Eames 1, Changzhu Jin 3, Ashli C. Everstine 4, Jason P. Sheehan 2, Beatriz S. Lopes 5, Neal Kassell 1 1 Focused Ultrasound Foundation, Charlottesville, Virginia, USA; 2 Neurosurgery, University of …
Get Price2020-12-10 · About 85% of Canada's production is exported to the USA, Japan and Europe (Heath, 1991). In Western Europe, Germany, the Netherlands and the United Kingdom are the major methanol-producing countries, accounting for 7%, 3% and over 2% of the world capacity, respectively (SRI, 1992).
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Get Price2021-2-3 · A Japanese study showed a protective effect for cardiovascular disease, but only in men. 47 By contrast, in a cohort of 117 366 Chinese men and women, 40-74 years of age, with a mean follow-up of 9.8 years and 309 events, Yu et al reported a hazard ratio of 1.80 (95% confidence interval 1.01 to 3.17) for highest versus lowest quarter of intake ...
Get Price2016-12-28 · The SW-846 Compendium consists of over 200 analytical methods for sampling and analyzing waste and other matrices. The methods are divided into sections, or “series,” according to the type of method, analyte (s) and technique (s) used. For more information on which method (s) to use, EPA developed a searchable table of all methods in the SW ...
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Get PriceThe s)llltem includes a complex system - ba.ed on the river mUe .yetem - for identifying sampling location. on all rivers and streams in the United State.. A recent addition to the .ystem introdllCes a location procedure baaed on geolraphic coordinates; this procedure is especir.lly ad8Pted to location of sampling sta..tbns in large bodies of ...
Get PriceProduct Support. Find support for your instrument including instruction manuals, software updates, spare parts, and repair request forms. Find information on Thermo Fisher Connect analysis apps, instrument management, data storage and security, and collaboration tools. Find self-help for questions about laboratory and industry-specific ...
Get PricePerforming challenge tests on RTE products should be highly recommended, also in order to generate data for the production and validation of predictive models, as valuable tool to be used in risk analysis activities. 5 CULTURE MEDIA. Aloa (Agar Listeria according to Ottaviani and Agosti) (BD Difco Oxford, United Kingdom).
Get PriceThe qPCR analysis was performed on a Bio-Rad CFX96 Touch (Bio-Rad, Hercules, CA, United States) in a 25 μL reaction volume. Each reaction consisted of 12.5 μL TaqMan Environmental Master Mix 2.0 (Thermo Fisher Scientific, Waltham, United States), 500 nM of each primer, 250 nM probe, nuclease free water and 5 μl DNA sample.
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Get Price2021-7-26 · Selected publications: Masuku B, Mkhwanazi N, Young E, Koch A, Warner DF. (2018) Beyond the lab: Eh!woza and knowing tuberculosis. Med Humanit. 44:285-292.. Moosa A, Lamprecht DA, Arora K, Barry CE 3rd, Boshoff HIM, Ioerger TR, Steyn AJC, Mizrahi V, Warner DF. (2017) Susceptibility of Mycobacterium tuberculosis cytochrome bd oxidase mutants to …
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Get Price2007-11-5 · USDA Database for the Oxygen Radical Absorbance Capacity (ORAC) of selected Foods S. Bhagwat, D.B.Haytowitz, and J.M.Holden Introduction Some hypotheses propose the benefits of antioxidant-rich diet.
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Get PriceDate of entry into force unknown (pending notification) or not yet in force., Date of effect: 01/01/1001; OJ L 67, 13.3.1999, p. 1–44 (ES, DA, DE, EL, EN, FR, IT, NL, PT, FI, SV) Special edition in Czech: Chapter 11 Volume 031 P. 40 - 83 Special edition in Estonian: Chapter 11 Volume 031 P. 40 - 83 Special edition in Latvian: Chapter 11 Volume 031 P. 40 - 83 Special edition in Lithuanian ...
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Get Price2016-12-19 · Natural hazards have the potential to trigger complex chains of events in technological installations leading to disastrous effects for the surrounding population and environment. The threat of climate change of worsening extreme weather events exacerbates the need for new models and novel methodologies able to capture the complexity of the natural …
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