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  • Lab Equipment and Lab Supplies | Fisher Scientific

    Epredia™ AlcoSCRUB™ Instant Antiseptic Hand Cleanser Squeeze Bottle. Ethyl Alcohol Non UV, MilliporeSigma™. MilliporeSigma™ Supelco™ BIOshell™ IgG C18 HPLC Column, 1,000 Å, 2.7 μm Particle Size. Honeywell Uvex™ Fog Eliminator Plus Cloths. Fisherbrand™ SterilElite 40 L …

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  • SOLUTIONS FOOD&FEED - Fisher Sci

    2021-7-27 · Simply position the capsule in the autosampler, enter sample name, type and weight and select the method and the calibration curve. Automatically, the software will set the analytical conditions according to the entered data. The dosing of gases is optimized by the software, in order to achieve complete combustion of the sample with minimum consumption.

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  • Fisher’s exact test from scratch with Python | by Tobias ...

    2020-2-2 · Fisher introduced his test by the “Lady tasting tea”- experiment³: At a tea party, a female colleague of Fisher’s claimed that she could distinguish whether tea or milk was added to the cup first. Fisher proposed to test the lady’s hypothesis with an experiment. He prepared eight cups of tea, four of each with milk or tea first.

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  • Reliable extraction of DNA from Whatman FTA cards -

    2021-7-25 · dried sample spot. Place the disc in a clean RNase/DNase-free 1.5 ml microcentrifuge tube. 2. Add 1 ml of PBS and centrifuge at 16 000 × g. 3. Macerate the disc using a 20-gauge syringe needle. 4. Centrifuge for 10 sec at 2000 × g. 5. Add 50 µl of buffer 1 to each sample. 6. Add 10 µl of Proteinase K to each sample and vortex for 15 sec. 7.

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  • Histology, Cytology, and Anatomical Pathology -

    In the embedding process, tissue samples and liquid embedding material are placed into molds and allowed to harden. The hardened blocks containing the tissue samples are then ready to be sectioned. Embedding can also be accomplished using frozen, non-fixed tissue in a water-based medium, usually a water-based glycol or resin. Differentiators

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  • Standard Methods for the Examination of Water ... -

    2021-7-26 · E. Ammonia-Selective Electrode Method Using Known Addition F. Phenate Method G. Automated Phenate Method H. Flow Injection Analysis 4500-NO 2‾ NITROGEN (NITRITE) A. Introduction B. Colorimetric Method 4500-NO 3‾ NITROGEN (NITRATE) A. Introduction B. Ultraviolet Spectrophotometric Screening Method

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  • LESSONS LEARNED FROM THE FIRST FULL CUP WET

    2019-1-10 · EGA sequence (i.e. without derivatization), the cup being used to accept sample is preconditioned and cleaned before use by heating to 900 C under He flow in the pyrolysis oven. Since MTBSTFA pyrolyzes at 900°C and wet chemistry cups are sealed, this cleaning step is non tenable.

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  • Thermo Scientific | Thermo Fisher Scientific - US

    Thermo Scientific instruments, equipment, software, services and consumables empower scientists to solve for complex analytical challenges in pharmaceutical, biotechnology, academic, government, environmental and industrial research, as well as the clinical laboratory. Our products address a range of needs from sample, material characterization and ...

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  • Optimization, performance, and application of a

    2018-7-27 · First, ten analysis cups without plastic, hereafter referred as “blank,” were pyrolyzed. For each blank and at the retention time of each characteristic peak of the eight used polymers (Table 1), the maximum height was determined over a time interval equal to 20 times the full width at half maximum (FWHM); this area is called H 20FWHM. Interval surrounds the retention time of each peak with the …

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  • Shop Products | Fisher Healthcare

    Verax Biomedical™ Platelet PGD™ Test System. Sterilis Solutions™ Regulated Medical Waste (RMW) Autoclave/Grinding System. Meridian Bioscience™ Revogene™ Instrument. Thermo Scientific™ M4RT Without Beads, NP Swab. Quidel Sofia 2 Flu+SARS Antigen FIA (25T) Siemens Healthineers Epoc™ Reader, Power Supply Included.

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  • Lab Equipment and Lab Supplies | Fisher Scientific

    Your source for innovative, indispensable lab equipment, lab supplies, and services. View our wide selection of products for scientific research and education.

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  • Reliable extraction of DNA from Whatman FTA cards -

    2021-7-25 · 12. Dissolve the pellet in 50 µl of TE buffer or water. DNA isolation using Chelex 100 resin Prepare a 5% w/v suspension of Chelex 100 resin in sterile water prior to use; stir to maintain a suspension. 1. Use a Harris Uni-Core disposable punch (1.2, 2.0, 3.0, or 6.0 mm) to remove a sample disc from the center of a dried sample spot.

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  • Guide to Measuring Fuel Dilution - Spectro Sci

    2017-11-9 · The most widely accepted direct method for analyzing fuel dilution in lubricants is Gas Chromatography (GC) according to ASTM methods D3524, D3525, and more recently, D7593.4-6 This method involves injecting a portion of an oil sample into a gas chromatograph. The GC vaporizes the sample and passes it through an analytical column

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  • Histology, Cytology, and Anatomical Pathology -

    Histology. Histology is the study of the microscopic anatomy of cells and tissues. Fisher Scientific offers a wide range of chemicals and reagents, including histology-grade solvents, designed specifically for medical laboratory professionals. All Fisher Scientific chemicals undergo rigorous quality assurance and testing procedures.

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  • Fisher’s exact test from scratch with Python | by Tobias ...

    2020-2-2 · Fisher’s exact test is an alternative to Pearson’s chi-squared test for independence. While actually valid for all sample sizes, Fisher’s exact test is practically applied when sample sizes are small. A general recommendation is to use Fisher’s exact test- instead of the chi-squared test - whenever more than 20 % of cells in a ...

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  • INSTRUCTIONS DNase I, RNase-free - Thermo Fisher

    2018-6-28 · Protein Sci 8(9): 1780-8. 9. Woame, I., et al. (2000). Irreversible heat inactivation of DNase I without RNA degradation. BioTechniques 29: 252-6. Products are warranted to operate or perform substantially in conformance with published Product specifications …

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  • Sci-Hub: removing barriers in the way of science

    The Sci-Hub project supports Open Access movement in science. Research should be published in open access, i.e. be free to read. The Open Access is a new and advanced form of scientific communication, which is going to replace outdated subscription models.

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  • The INTACT method for cell type–specific gene

    2010-12-16 · A high yield of undamaged nuclei can be obtained from many tissues by using the freezing and grinding method presented here, but if problems arise with obtaining good nuclei from the tissue of ...

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  • Wear and Creep of Highly Crosslinked Polyethylene

    2010-4-27 · The wear and creep characteristics of highly crosslinked ultrahigh-molecular-weight polyethylene (UHMWPE) articulating against large-diameter (36 mm) ceramic and cobalt chrome femoral heads have been investigated in a physiological anatomical hip joint simulator for 10 million cycles.

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  • All-in-one sequencing: an improved library

    2020-5-24 · Flowchart for the All-in-One sequencing (AIO-seq) method. a The libraries were prepared using Tn5 transposase.b The process of mechanical fragmentation was used to prepare the libraries.c In the traditional protocol, the size selection, and quantification were processed using a one sample one tube method.d With the AIO-seq method, the library analyzed by the Agilent 2100 Bioanalyzer will give ...

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  • Food Analysis - Fisher Sci

    2021-7-7 · • Randall method: 1) Sample extraction by immersing the sample cartridge in boiling solvent 2) Rinsing the sample in a cooled, recondensed solvent 3) Solvent recovery by distillation and collection of the condensed vapours in the upper part. three or six extraction cups …

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  • Guide to Measuring Fuel Dilution - Spectro Sci

    2017-11-9 · The most widely accepted direct method for analyzing fuel dilution in lubricants is Gas Chromatography (GC) according to ASTM methods D3524, D3525, and more recently, D7593.4-6 This method involves injecting a portion of an oil sample into a gas chromatograph. The GC vaporizes the sample and passes it through an analytical column

    Get Price
  • Fisher’s exact test from scratch with Python | by Tobias ...

    2020-2-2 · Fisher’s exact test is an alternative to Pearson’s chi-squared test for independence. While actually valid for all sample sizes, Fisher’s exact test is practically applied when sample sizes are small. A general recommendation is to use Fisher’s exact test- instead of the chi-squared test - whenever more than 20 % of cells in a ...

    Get Price
  • Evaluation method for asymmetric uncertainty of ...

    2021-6-2 · The dilution buffer was prepared by mixing 1480 μL of 1× TE buffer (TE, pH 7.0, RNase-free, Thermo Fisher Scientific), 1480 μL of UltraPure DNase/RNase-Free Distilled Water (Thermo Fisher ...

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  • INSTRUCTIONS DNase I, RNase-free - Thermo Fisher

    2018-6-28 · Protein Sci 8(9): 1780-8. 9. Woame, I., et al. (2000). Irreversible heat inactivation of DNase I without RNA degradation. BioTechniques 29: 252-6. Products are warranted to operate or perform substantially in conformance with published Product specifications …

    Get Price
  • DNAZOL Reagent - Fisher Sci

    2018-9-7 · During the isolation, a biological sample is lysed (or homogenized) in DNAZOL Reagent and the genomic DNA is precipitated from the lysate with ethanol. Following an ethanol wash, DNA is solubilized in water or 8 mM NaOH. The procedure can be completed in 10-30 min with DNA recovery of 70-100%. The isolated DNA can be used without

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  • Feasibility of Manufacturing Disposable Cups using

    2021-2-19 · The PCL/WP concentrates were prepared by the melt intercalation method, using a high-speed mixer (Thermokinetic Homogenizer), model MH-50H (MH equipment, Guarulhos, Brazil). The thermokinetic homogenizer consists of a horizontal mixer, without heating source, with cooling system and cylindrical mixing chamber and shaft with high-speed ...

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  • Wear and Creep of Highly Crosslinked Polyethylene

    2010-4-27 · The wear and creep characteristics of highly crosslinked ultrahigh-molecular-weight polyethylene (UHMWPE) articulating against large-diameter (36 mm) ceramic and cobalt chrome femoral heads have been investigated in a physiological anatomical hip joint simulator for 10 million cycles.

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  • A streamlined clinical metagenomic sequencing

    2021-2-23 · For sample #1 with a Ct value of 19.02, near full-length H1N1 viral genomes (98.75%) and an average depth of 13.84 were obtained (Table 3). Table …

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  • Substituting HF by HBF4 – an optimized digestion

    2020-7-24 · An additional advantage of using HBF 4 is that excess fluoride ions are directly complexed by H 3 BO 3 and HBF 3 OH thus preventing precipitation of metal fluorides. 25,29 This allows the direct analysis of the samples without any further measures, which helps to speed up sample preparation, as well as to avoid errors e.g. due to contamination ...

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  • Evaluation method for asymmetric uncertainty of ...

    2021-6-2 · The dilution buffer was prepared by mixing 1480 μL of 1× TE buffer (TE, pH 7.0, RNase-free, Thermo Fisher Scientific), 1480 μL of UltraPure DNase/RNase-Free Distilled Water (Thermo Fisher ...

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  • Filtration for Air Monitoring - Fisher Scientific

    2021-7-27 · Whatman TE type and PM 2.5 are chemically resistant and possess low chemical background interference (e.g., metals), allowing the user to carry out sensitive determinations. Whatman TE membranes are general purpose PTFE membrane filters that have multiple applications in environmental analysis. PM 2.5 filters are used for the measurement of fine

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  • Feasibility of Manufacturing Disposable Cups using

    2021-2-19 · Use of fiber reinforced composites derived from renewable sources has increased in recent years, aiming at a balance between sustainability and technological development. This research sought to develop poly (lactic acid) (PLA)/poly (ε-caprolactone) (PCL) composites using wood powder (WP) as reinforcement. The composites were initially prepared in a co‐rotational twin‐screw extruder, and ...

    Get Price
  • A novel method for the prediction of functional

    2001-2-1 · A hip joint simulator using new and physiologically scratched femoral heads with ultra-high molecular weight polyethylene acetabular cups Proc. Instn Mech. Engrs, Part H, Journal of Engineering in Medicine, 2000, 214 (H6), 569 – 576.

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  • Antibodies & Protein Biology - Fisher Scientific

    1. Beckman Coulter Multisizer™ 4e Coulter Counter. The Multisizer 4e Coulter Counter uses the Coulter Principle to detect particles via electrical zone sensing, regardless of the particle’s nature or optical properties. Therefore, the Coulter Counter is an ideal tool for …

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  • A semi-target analytical method for quantification of

    2019-7-29 · Hydroxylated polychlorinated biphenyls (OH-PCBs) are oxidative metabolites of PCBs and residuals found in original Aroclors. OH-PCBs are known to play a role as genotoxicants, carcinogens, and hormone disruptors, and therefore it is important to quantify their presence in human tissues, organisms, and environmental matrices. Of 837 possible mono-OH-PCBs congeners, there are only ~ …

    Get Price
  • Dimeric Cyanine Nucleic Acid Stains - Thermo Fisher

    2005-10-22 · dure is adapted from a published experiment using SYBR Green I nucleic acid gel stain 14 (S-7563, S-7567), but should be gener-ally applicable for use with any of the dimeric cyanine dyes. 1.1 Dilute the stain 10,000-fold in TE buffer (10 mM Tris, 1 mM EDTA, pH 8.0) or TBE buffer (45 mM Tris-borate, 1 mM EDTA, pH 8.0).

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  • Syringe-injectable mesh electronics integrate

    2017-6-6 · Images of flexible thin-film probes were acquired using the same method. Confocal images from both sides of the antibody-labeled sagittal slice in Fig. 4 were acquired using the same excitation sources as above, a 3-AU pinhole, which yields an optical section thickness of ca. 9 μm, and image planes centered at ca. 5 μm below surfaces of the ...

    Get Price
  • Sci-Hub

    The Sci-Hub project supports Open Access movement in science. Research should be published in open access, i.e. be free to read. The Open Access is a new and advanced form of scientific communication, which is going to replace outdated subscription models.

    Get Price
  • eBioscience™ Fixable Viability D ye eFluor ... - Fisher Sci

    2018-8-15 · experiments using live, unfixed cells. 6. For compensation, it is recommended to use a sample of the cells of interest stained with the FVD only. If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute, then immediately place on ice for 1 minute.

    Get Price
  • Feasibility of Manufacturing Disposable Cups using

    2021-2-19 · Use of fiber reinforced composites derived from renewable sources has increased in recent years, aiming at a balance between sustainability and technological development. This research sought to develop poly (lactic acid) (PLA)/poly (ε-caprolactone) (PCL) composites using wood powder (WP) as reinforcement. The composites were initially prepared in a co‐rotational twin‐screw extruder, and ...

    Get Price
  • ExpiCHO Expression System - Fisher Sci

    2020-10-20 · Using a 2-mL or 5-mL pipette, transfer the entire contents of the cryovial into a 125-mL polycarbonate, disposable, sterile, vent-cap Erlenmeyer shaker flask containing 30 mL of …

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  • A new method for determining gastric acid output

    2013-5-3 · Assuming that correlation would range between 0.5 and 0.7 between methods, a sample size of 20 subjects would allow for acceptable confidence intervals. Correlations were calculated using Pearson's correlation analysis and anova except in the case of SP emptying time vs. GES and SP GAO vs. BAO, where Spearman's ranked correlation and anova were ...

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  • AQUALAB 4TE | Accurate and reliable Water Activity

    2001-2-1 · A hip joint simulator using new and physiologically scratched femoral heads with ultra-high molecular weight polyethylene acetabular cups Proc. Instn Mech. Engrs, Part H, Journal of Engineering in Medicine, 2000, 214 (H6), 569 – 576.

    Get Price
  • A novel method for the prediction of functional

    2018-6-28 · Protein Sci 8(9): 1780-8. 9. Woame, I., et al. (2000). Irreversible heat inactivation of DNase I without RNA degradation. BioTechniques 29: 252-6. Products are warranted to operate or perform substantially in conformance with published Product specifications …

    Get Price
  • INSTRUCTIONS DNase I, RNase-free - Thermo Fisher

    2010-3-2 · The fine detail provided by sequencing-based transcriptome surveys suggests that RNA-seq is likely to become the platform of choice for interrogating steady state RNA. In order to discover biologically important changes in expression, we show that normalization continues to be an essential step in the analysis. We outline a simple and effective method for performing normalization and show ...

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  • A scaling normalization method for differential

    2021-7-18 · RNA can be isolated in ≤ 1 hour. The sample can be purified using two methods, which include microcentrifugation (spin) and vacuum. It uses a simple and fast procedure. The procedure includes a DNase treatment step to reduce genomic DNA contamination, without any DNase carryover in the final purified RNA.

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  • Product Brochure Scepter 2.0 Cell Counter - Fisher Sci

    2021-7-9 · • Compare sample sets side by side using histogram ... (CV) for each counting method shown was calculated using cell concentration measurements at 50,000 cells/mL samples of 19 different cell lines. The Scepter™ cell ... Te 3 3.124e5 mL Jun 11 20:45:13 49 300 2.22pL 16.19µm

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  • TR0027 Elution Conditions - Thermo Fisher Scientific - US

    2009-7-7 · captured target molecules are released and recovered (i.e., eluted) from the ligand molecules using buffer conditions that disrupt the affinity interaction. Appropriate buffer conditions for binding and elution steps in affinity purification are as varied as the types of molecules concerned and their chemical binding properties.

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  • eBioscience™ Fixable Viability D ye eFluor ... - Fisher Sci

    2018-8-15 · experiments using live, unfixed cells. 6. For compensation, it is recommended to use a sample of the cells of interest stained with the FVD only. If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute, then immediately place on ice for 1 minute.

    Get Price
  • (PDF) Determining Sample Size; How to Calculate

    2017-6-6 · Images of flexible thin-film probes were acquired using the same method. Confocal images from both sides of the antibody-labeled sagittal slice in Fig. 4 were acquired using the same excitation sources as above, a 3-AU pinhole, which yields an optical section thickness of ca. 9 μm, and image planes centered at ca. 5 μm below surfaces of the ...

    Get Price
  • Syringe-injectable mesh electronics integrate

    2019-5-14 · The importance of early cancer diagnosis and improved cancer therapy has been clear for years and has initiated worldwide research towards new possibilities in the care strategy of patients with cancer using technological innovations. One of the key research fields involves the separation and detection of circulating tumor cells (CTC) because of their suggested important role in early cancer ...

    Get Price
  • Technologies for circulating tumor cell separation

    2019-4-23 · DNA methylation is a chemically stable yet biologically dynamic mark ().A family of DNA methyltransferases (DNMTs) catalyzes the conversion of cytosine to 5-methylcytosine ().Maintenance of DNA methylation during cell division is a regulated process involving the DNA methyltransferase Dnmt1 and its regulatory adapter protein Uhrf1, among other molecules (9, 10).

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  • A Practical Guide to the Measurement and Analysis of

    2020-6-30 · Above 650 °C the Te is likely vaporized too quickly to effectively react with the Pt, or any PtTe 2 that does form then loses Te to the atmosphere. This hypothesis is supported by previous work on MoTe 2 synthesis using a similar method and by the reports of telluride TMDs losing Te at elevated temperature in Te-deficient environments [40, 44 ...

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  • Low-temperature synthesis and electrocatalytic

    2020-3-17 · Using the company’s estimates, calculate the percentage of policyholders that will renew at least one policy next year. (A) 20% (B) 29% (C) 41% (D) 53% (E) 70% . 8. Among a large group of patients recovering from shoulder injuries, it is found that 22% visit both a physical therapist and a chiropractor, whereas 12% visit neither of these. The

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  • EXAM P SAMPLE QUESTIONS - SOA

    2016-9-21 · teaches power analysis using Monte Carlo simulation is about to be pub­ lished (Borenstein, M. & Cohen, J., 1988). It would seem that power analysis has arrived. Yet recently, two independent investigations have come to my attention that give me pause. Rossi, Rossi, and Cottril (in press), using …

    Get Price
  • Product Brochure Scepter 2.0 Cell Counter - Fisher Sci

    2021-7-9 · • Compare sample sets side by side using histogram ... (CV) for each counting method shown was calculated using cell concentration measurements at 50,000 cells/mL samples of 19 different cell lines. The Scepter™ cell ... Te 3 3.124e5 mL Jun 11 20:45:13 49 300 2.22pL 16.19µm

    Get Price
  • TR0027 Elution Conditions - Thermo Fisher Scientific - US

    2009-7-7 · captured target molecules are released and recovered (i.e., eluted) from the ligand molecules using buffer conditions that disrupt the affinity interaction. Appropriate buffer conditions for binding and elution steps in affinity purification are as varied as the types of molecules concerned and their chemical binding properties.

    Get Price
  • A semi-target analytical method for quantification of

    2019-7-29 · Hydroxylated polychlorinated biphenyls (OH-PCBs) are oxidative metabolites of PCBs and residuals found in original Aroclors. OH-PCBs are known to play a role as genotoxicants, carcinogens, and hormone disruptors, and therefore it is important to quantify their presence in human tissues, organisms, and environmental matrices. Of 837 possible mono-OH-PCBs congeners, there are only ~ …

    Get Price
  • Low-temperature synthesis and electrocatalytic

    2020-6-30 · Above 650 °C the Te is likely vaporized too quickly to effectively react with the Pt, or any PtTe 2 that does form then loses Te to the atmosphere. This hypothesis is supported by previous work on MoTe 2 synthesis using a similar method and by the reports of telluride TMDs losing Te at elevated temperature in Te-deficient environments [40, 44 ...

    Get Price
  • Technologies for circulating tumor cell separation

    2019-5-14 · The importance of early cancer diagnosis and improved cancer therapy has been clear for years and has initiated worldwide research towards new possibilities in the care strategy of patients with cancer using technological innovations. One of the key research fields involves the separation and detection of circulating tumor cells (CTC) because of their suggested important role in early cancer ...

    Get Price
  • VWR, Part of Avantor - Chemicals and Laboratory

    VWR, Part of Avantor - Chemicals and Laboratory Scientific Supplies. VWR, Part of Avantor. Carousel. Moving Forward. Together. So much has changed during this unprecedented time, except your ability to count on Avantor. We continue to set science in motion, and know safety is …

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  • Syringe-injectable mesh electronics integrate

    2017-6-6 · Images of flexible thin-film probes were acquired using the same method. Confocal images from both sides of the antibody-labeled sagittal slice in Fig. 4 were acquired using the same excitation sources as above, a 3-AU pinhole, which yields an optical section thickness of ca. 9 μm, and image planes centered at ca. 5 μm below surfaces of the ...

    Get Price
  • A Practical Guide to the Measurement and Analysis of

    2019-4-23 · DNA methylation is a chemically stable yet biologically dynamic mark ().A family of DNA methyltransferases (DNMTs) catalyzes the conversion of cytosine to 5-methylcytosine ().Maintenance of DNA methylation during cell division is a regulated process involving the DNA methyltransferase Dnmt1 and its regulatory adapter protein Uhrf1, among other molecules (9, 10).

    Get Price
  • CancerLocator: non-invasive cancer diagnosis and

    2017-3-24 · We propose a probabilistic method, CancerLocator, which exploits the diagnostic potential of cell-free DNA by determining not only the presence but also the location of tumors. CancerLocator simultaneously infers the proportions and the tissue-of-origin of tumor-derived cell-free DNA in a blood sample using genome-wide DNA methylation data.

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  • Influence of different extracts addition on total phenols ...

    extracts using different methods showing that same extract reacted differently with different reagents depending on used method. Table 2. Antioxidant activity (mmol TE/100 mL) of blackberry juice without and with addition of extracts during 52 days of storage at 4 °C Antioxidant activity (mmol TE/100 mL) Sample DPPH method ABTS method

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  • Low-temperature synthesis and electrocatalytic

    2020-6-30 · Above 650 °C the Te is likely vaporized too quickly to effectively react with the Pt, or any PtTe 2 that does form then loses Te to the atmosphere. This hypothesis is supported by previous work on MoTe 2 synthesis using a similar method and by the reports of telluride TMDs losing Te at elevated temperature in Te-deficient environments [40, 44 ...

    Get Price
  • Lowering the quantification limit of the Qubit TM RNA

    2015-5-6 · The lower reading limit of the Qubit™ RNA HS assay for accurate quantification is 25 pg/μL (deviation from ideal < 20%). In order to quantify samples that fall below this limit, prior to adding the RNA sample that was to be measured, we added 5 ng of RNA spike-in (2.5 ng/μL Qubit™ RNA standard #2) into a 200 μL Qubit™ assay to generate an expected baseline reading of 25 pg/μL.

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  • A simple method to estimate the average localization ...

    2011-7-1 · The collecting cups were removed and kept in a hot-air oven at 40°C for 30 minutes to drive out any traces of petroleum ether. From the weights of, empty collecting cups, cups with oil, and initial ground peanut kernel meal, the total oil percentage was calculated. The measurements were done in triplicate for each cultivar (Sundaram et al ...

    Get Price
  • Nondestructive NIR Reflectance Spectroscopic

    2017-3-24 · We propose a probabilistic method, CancerLocator, which exploits the diagnostic potential of cell-free DNA by determining not only the presence but also the location of tumors. CancerLocator simultaneously infers the proportions and the tissue-of-origin of tumor-derived cell-free DNA in a blood sample using genome-wide DNA methylation data.

    Get Price
  • CancerLocator: non-invasive cancer diagnosis and

    2020-3-17 · Using the company’s estimates, calculate the percentage of policyholders that will renew at least one policy next year. (A) 20% (B) 29% (C) 41% (D) 53% (E) 70% . 8. Among a large group of patients recovering from shoulder injuries, it is found that 22% visit both a physical therapist and a chiropractor, whereas 12% visit neither of these. The

    Get Price
  • EXAM P SAMPLE QUESTIONS - SOA

    240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 [email protected]

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  • SNAP-tag® Technologies: Tools to Study Protein

    2021-7-18 · RNA can be isolated in ≤ 1 hour. The sample can be purified using two methods, which include microcentrifugation (spin) and vacuum. It uses a simple and fast procedure. The procedure includes a DNase treatment step to reduce genomic DNA contamination, without any DNase carryover in the final purified RNA.

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  • RNA Extraction - labome.com

    2021-5-26 · Using multiple approaches, we provide evidence for an endogenous IHR mechanism in the early embryo that can be enhanced by RAD51. This process can be harnessed to generate homozygotes from wild-type zygotes using exogenous donors and to convert heterozygous alleles into homozygous alleles without exogenous templates.

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  • Discrimination of SARS-CoV-2 infected patient

    2021-4-14 · Urine from all subjects was collected via clean catch into sterile sample cups. Urine cups were provided by nursing staff during routine clinical care. For saliva samples, participants under 18 years of age were requested to tip the cotton ball (CURAD Sterile Cotton Balls, 1” or 2.54 cm) into their mouth without touching it with their hands.

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  • eBioscience™ Fixable Viability D ye eFluor ... - Fisher Sci

    2018-8-15 · experiments using live, unfixed cells. 6. For compensation, it is recommended to use a sample of the cells of interest stained with the FVD only. If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute, then immediately place on ice for 1 minute.

    Get Price
  • SciELO - Brasil - Comparative evaluation of patellar ...

    One hundred and ninety-six randomly selected patients were included in the sample. RESULTS: The data were initially evaluated using the chi-square test. This analysis was deemed to be positive with p < 0.0001. We compared the traditional methods with the plateau-patella angle measurement, using Fisher…

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  • Nondestructive NIR Reflectance Spectroscopic

    2011-7-1 · The collecting cups were removed and kept in a hot-air oven at 40°C for 30 minutes to drive out any traces of petroleum ether. From the weights of, empty collecting cups, cups with oil, and initial ground peanut kernel meal, the total oil percentage was calculated. The measurements were done in triplicate for each cultivar (Sundaram et al ...

    Get Price
  • A simple method to estimate the average localization ...

    2014-8-17 · X-ray fluorescence spectroscopy (XRF) has been used extensively to analyze many types of environmental samples, including lake sediments. In most cases, however, analyses have required either a relatively large sample mass or sample pretreatment, e.g. lithium borate fusion, and have not taken advantage of the potential of XRF analysis as a non-destructive technique. This paper describes the ...

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  • Wavelength dispersive X-ray fluorescence

    2016-9-21 · teaches power analysis using Monte Carlo simulation is about to be pub­ lished (Borenstein, M. & Cohen, J., 1988). It would seem that power analysis has arrived. Yet recently, two independent investigations have come to my attention that give me pause. Rossi, Rossi, and Cottril (in press), using …

    Get Price
  • Statistical Power Analysis for the Behavioral Sciences

    Pd-intercalated RTe n specimens are synthesized using solid-state reaction techniques at moderate temperatures by repeatedly grinding together and heating product in a vacuum-sealed quartz ampule to 500–700 °C for 50 h, or a Te self-flux method at higher temperatures (800–900 °C) by adding Pd to the melted product and slowly cooling over ...

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  • Advances in Rare‐Earth Tritelluride Quantum Materials ...

    The direct synthesis of hydrogen peroxide is investigated using PdGa/TiO 2 and PdIn/TiO 2 catalysts prepared by an acid-washed sol-immobilisation procedure, which allows for good control of particle size. The introduction of both Ga and In into a supported Pd catalyst leads to significantly reduced rates of H 2 O 2 degradation in comparison to the monometallic counterpart.

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  • Menstrual cup use, leakage, acceptability, safety, and ...

    Menstrual cups are made of medical-grade silicone, rubber, latex, or elastomer and can last up to 10 years; disposable single-use menstrual cups also exist. We aimed to summarise current knowledge about leakage, safety, and acceptability of menstrual cups and …

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  • Optimization of a high-throughput CTAB-based

    2011-12-1 · Standard method in 2.0-mL microcentrifuge tubes: The original samples used in all the protocols described herein consisted of 1.8 mL of rumen fluid and 50 mg of ground plant seed material. Samples were lyophilized at − 40 °C for 48 h and bead-beaten on a prechilled rack at − 80 °C for 1 min using 8-mm glass beads (Fisher).

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  • Comparative evaluation of methods for isolating small ...

    2021-2-10 · The sample was incubated under boiling water for 5 min, and then cooled to room temperature. 200 μL of UA buffer (8M Urea, 150mM Tris-HCl, pH 8.5) was added to the sample, followed by centrifuging at 12,500 g for 15 min using a 30 kDa ultrafiltration tube …

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  • Automated High-Throughput Metabolic Stability

    2019-5-14 · The importance of early cancer diagnosis and improved cancer therapy has been clear for years and has initiated worldwide research towards new possibilities in the care strategy of patients with cancer using technological innovations. One of the key research fields involves the separation and detection of circulating tumor cells (CTC) because of their suggested important role in early cancer ...

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  • Technologies for circulating tumor cell separation

    TF-1 cells were plated in growth media without antibiotics at 30,000 cells per well in a white 96-well assay plate and transfected with a CMV-luc2 plasmid using various lipid (ViaFect™ Transfection Reagent):DNA ratios. The DNA concentration was held constant at 1μg per 100μl of Opti-MEM® I reduced-serum medium, and the amount of ViaFect ...

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  • Transfection Guide | Overview of Transfection

    2018-8-27 · Immune protection in newborn calves relies on a combination of the timing, volume and quality of colostrum consumed by the calf after birth. Poor quality colostrum with inadequate immunoglobulin concentration contributes to failed transfer of passive immunity in calves, leading to higher calf morbidity and mortality. Therefore, estimating colostrum quality and ensuring the transfer …

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  • An application of PCR-RFLP species identification

    2011-5-6 · Preparation. • Punch a small hole in center of the bottom of each cup (for plastic cups, you might need a nail or other sharp tool, so use caution when completing this step). • Thread one end ...

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